Modeling Beta-Traces for Beta-Barrels from Cryo-EM Density Maps
نویسندگان
چکیده
منابع مشابه
Modeling Beta-Traces for Beta-Barrels from Cryo-EM Density Maps
Cryo-electron microscopy (cryo-EM) has produced density maps of various resolutions. Although α-helices can be detected from density maps at 5-8 Å resolutions, β-strands are challenging to detect at such density maps due to close-spacing of β-strands. The variety of shapes of β-sheets adds the complexity of β-strands detection from density maps. We propose a new approach to model traces of β-st...
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Major secondary structure elements such as α helices and β sheets can be computationally detected from cryoelectron microscopy (cryo-EM) density maps with medium resolutions of 5-10 Å. However, a critical piece of information for modeling atomic structures is missing, because there are no tools to detect β strands from cryo-EM maps at medium resolutions. We propose a method, StrandTwister, to d...
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Cryo-electron microscopy (Cryo-EM) is a powerful technique to produce 3-dimensional density maps for large molecular complexes. Although many atomic structures have been solved from cryo-EM density maps, it is challenging to derive atomic structures when the resolution of density maps is not sufficiently high. Geometrical shape representation of secondary structural components in a medium-resol...
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NATURE METHODS | VOL.11 NO.1 | JANUARY 2014 | 63 critical because local resolution tests are repeated at many points in the volume. Additionally, there is data dependency between neighboring points that windowed FSC does not account for. We propose a mathematical theory and an efficient algorithm for measuring local resolution that address all of the above limitations. The theory (Online Method...
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ژورنال
عنوان ژورنال: BioMed Research International
سال: 2017
ISSN: 2314-6133,2314-6141
DOI: 10.1155/2017/1793213